GST融合蛋白纯化磁珠

海狸GST融合蛋白纯化磁珠是专为高效、快速纯化谷胱甘肽S-转移酶(GST)融合蛋白而设计的一种新型功能化材料，可通过磁性分离方式直接从生物样品中一步纯化出高纯度的目标蛋白，极大地简化纯化工艺和提高纯化效率，适合科研和工业领域便捷地进行GST融合蛋白的纯化。
与传统的柱层析纯化方式相比，采用海狸磁珠纯化GST融合蛋白，无需对粗蛋白样品进行多次长时间的高速离心，也不需要过滤操作；无需控制流速，更不需要昂贵的层析设备。样品与磁珠的特异性结合、洗涤和目标蛋白洗脱变得非常简单、快速、易操作，不存在样品流速控制的瓶颈，对于熟练的操作者来说，在一个小时内就能获得高纯度 的目的蛋白，不仅为研究者简化了流程，节省了时间，降低了成本，并可提高目标蛋白的纯化效率。使用磁珠能轻 松实现多样品平行处理，实现高通量，也可任意放大样品纯化规模。

应用实例

1. 磁珠与传统层析柱纯化GST融合蛋白对比

2.平行操作稳定性高，可方便实现高通量和大规模蛋白纯化

结果：CV(目标蛋白纯度） = 1.6%，CV（ 目标蛋白载量）= 4.5%。 此外，放大规模纯化样品时，只需同比例增加磁珠用量即可，并不影响目标蛋白的产率和纯度，完全不存在流速和样品体积的限制，海狸磁珠更加适用于大规模蛋白纯化生产。

3.不同批次间产品使用性能稳定，批次间差异小

结果：CV(目标蛋白纯度）= 0.7%，CV（ 目标蛋白载量）= 4.7%

4.可重复使用

结果：同一样品，重复使用5次后，其目标蛋白载量没有明显下降，目标蛋白纯度基本不变。为了保障纯化的质量，建议3~5次后，进行清洗使用处理。

产品特性

1、蛋白纯化快捷

     操作简单，一步纯化即可得到高纯度蛋白；

     方便快捷，一个小时轻松实现蛋白纯化。

2、蛋白纯化灵活

     可同时处理多个样品，实现高通量；

     可轻松实现蛋白浓度和体积的控制，方便后续样品精制；

     可轻松实现对纯化规模的控制；

     对微量和低丰度样品具有良好的纯化效果。

3、蛋白纯化经济

     纯化流程短，蛋白产量及活性高；

     设备简单，减少了设备购买及维护成本，蛋白纯化门槛大大降低；

     纯化时间短，节约了时间成本；

     可同时进行多样品处理，实现自动化操作，节约人工成本；

     能够重复使用。

引用文献：

1. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae. Nature, 2023.

2. RPL21 interacts with LAMP3 to promote colorectal cancer invasion and metastasis by regulating focal adhesion formation. Cellular & molecular biology letters, 2023.

3. Symbiosis between Dendrobium catenatum protocorms and Serendipita indica involves the plant hypoxia response pathway. Plant Physiology, 2023.

4. Coupling of Slack and NaV1.6 sensitizes Slack to quinidine blockade and guides anti-seizure strategy development. bioRxiv : the preprint server for biology, 2023.

5. BPIFB2 is highly expressed in "cold" lung adenocarcinoma and decreases T cell chemotaxis via activation of the STAT3 pathway. Mol Cell Probes 2022 04;62.

6. Characterization of aspartyl aminopeptidase from Schistosoma japonicum. Acta Trop 2022 Aug;232.

7. Characterization of regulatory modules controlling leaf angle in maize. Plant Physiol 2022 Jun 27. DOI: 10.1093/plphys/kiac308/6618538.

8. Differential expressions and enzymatic properties of malate dehydrogenases in response to nutrient and metal stresses in Stylosanthes guianensis. Plant Physiol Biochem 2022 Jan 01;170.

9. Dwarf and High Tillering1 represses rice tillering through mediating the splicing of D14 pre-mRNA. Plant Cell 2022 Jun 07.

10. Genome-wide association study identifies variants of GhSAD1 conferring cold tolerance in cotton. J Exp Bot 2022 04 05;73(7).

11. Heat Shock Protein Member 8 (HSPA8) Is Involved in Porcine Reproductive and Respiratory Syndrome Virus Attachment and Internalization. Microbiol Spectr 2022 02 23;10(1).

12. Nitrate-inducible MdBT2 acts as a restriction factor to limit apple necrotic mosaic virus genome replication in Malus domestica. Mol Plant Pathol 2022 03;23(3).

13. Pantothenate kinase 2 interacts with PINK1 to regulate mitochondrial quality control via acetyl-CoA metabolism. Nat Commun 2022 05 03;13(1).

14. PGK1-coupled HSP90 stabilizes GSK3β expression to regulate the stemness of breast cancer stem cells. Cancer Biol Med 2021 Aug 17.

15. Proteomics analysis reveals a critical role for the WSSV immediate-early protein IE1 in modulating the host prophenoloxidase system. Virulence 2022 12;13(1).

16. The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance. Plant J 2022 May 02. 

17. Toward a Treatment of Cancer Design and In VitroIn Vivo Evaluation of Uncharged Pyrazoline Derivatives as a Series of Novel SHP2 Inhibitors. Int. J. Mol. Sci. 2022, 23, 3497.

18. Usp8 promotes tumor cell migration through activating the JNK pathway. Cell Death Dis 2022 Mar 31;13(3).

19. Coordinated cytokinin signaling and auxin biosynthesis mediates arsenate-induced root growth inhibition. Plant Physiology, 2021, 185(3): 1166-118. DOI: 10.1093/plphys/kiaa072.

20. The transcription factor PagLBD3 contributes to the regulation of secondary growth in Populus. Journal of Experimental Botany, Volume 72, Issue 20, 26 October 2021, Pages 7092–7106. DOI: 10.1093/jxb/erab351.

21. RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa. Biotechnology for Biofuels, 2021, 14(1): 1-17. DOI: 10.1186/s13068-021-01877-2.

22. Nitrate-inducible MdBT2 acts as a restriction factor to limit apple necrotic mosaic virus genome replication in Malus domestica. Molecular Plant Pathology, 2021. DOI: 10.1111/mpp.13166.

23. Naa10p and IKKα interaction regulates EMT in oral squamous cell carcinoma via TGF-β1/Smad pathway. Journal of Cellular and Molecular Medicine, 2021. DOI: 10.1111/jcmm.16680.

24. A Riemerella anatipestifer Metallophosphoesterase That Displays Phosphatase Activity and Is Associated with Virulence. Applied and Environmental Microbiology, 2021, 87(11): e00086-21. DOI: 10.1128/AEM.00086-21.

25. Wheat TaPUB1 protein mediates ABA response and seed development through ubiquitination. Plant Science, 2021, 309: 110913. DOI: 10.1016/j.plantsci.2021.110913.

26. SUV39H1 is a New Client Protein of Hsp90 Degradated by Chaetocin as a Novel C-Terminal Inhibitor of Hsp90. Biomolecules & Therapeutics, 2021, 29(1): 73. DOI: 10.4062/biomolther.2020.012.

27. Reduction of OsMPK6 activity by a R89K mutation induces cell death and bacterial blight resistance in rice. Plant Cell Reports, 2021, 40(5): 835-850. DOI: 10.1007/s00299-021-02679-4.

28. Differential expressions and enzymatic properties of malate dehydrogenases in response to nutrient and metal stresses in Stylosanthes guianensis. Plant Physiology and Biochemistry, 2021. DOI: 10.1016/j.plaphy.2021.12.012.

29. PGK1-coupled HSP90 stabilizes GSK3β expression to regulate the stemness of breast cancer stem cells. Cancer Biology & Medicine, 2021, 18(2): 1-25. DOI: 10.20892/j.issn.2095-3941.2020.0362.

30. A cysteine-rich receptor-like protein kinase CaCKR5 modulates immune response against Ralstonia solanacearum infection in pepper. BMC plant biology, 2021, 21(1): 1-15. DOI: 10.1186/s12870-021-03150-y.

31. CaWRKY28 Cys249 is Required for Interaction with CaWRKY40 in the Regulation of Pepper Immunity to Ralstonia solanacearum.  Molecular Plant-Microbe Interactions, 2021 (ja). DOI: 10.1094/MPMI-12-20-0361-R.

32. CaPti1/CaERF4 module positively regulates pepper resistance to bacterial wilt disease through coupling enhanced immunity and dehydration tolerance. bioRxiv, 2021. DOI: 10.1101/2021.10.26.465959.

33. EV71 病毒中和表位和诺如病毒P 结构域嵌合蛋白的原核表达；China Biotechnology，2017，37( 1) : 1-6, DOI: 10.13523 /j.cb.20170101.

34. Functional characterization of rice CW-domain containing zinc finger proteins involved in histone recognition；Plant Science 263 (2017) 168–176，DOI:10.1016/j.plantsci.2017.06.013.

35. The potassium channel FaTPK1 plays a critical role in fruit quality formation in strawberry (Fragaria× ananassa)；Plant Biotechnology Journal (2017), pp. 1–12，DOI: 10.1111/pbi.12824.

36. Identification of Interacting Motifs Between Armadillo Repeat Containing 1 (ARC1) and Exocyst 70 A1 (Exo70A1) Proteins in Brassica oleracea;PROTEIN JOURNAL  卷: 35   期: 1   页: 34-43   出版年: FEB 2016.

37. N-terminal domains of ARC1 are essential for interaction with the N-terminal region of Exo70A1 in transducing self-incompatibility of Brassica oleracea；ACTA BIOCHIMICA ET BIOPHYSICA SINICA  卷: 48   期: 9   页: 777-787   出版年: SEP 2016.

38. Orf Virus 002 Protein Targets Ovine Protein S100A4 and Inhibits NF-κB Signaling；FRONTIERS IN MICROBIOLOGY  卷: 7     文献号: 1389   出版年: SEP 13 2016.

39. Identification of Interacting Motifs Between Armadillo Repeat Containing 1 (ARC1) and Exocyst 70 A1 (Exo70A1) Proteins in Brassica oleracea；PROTEIN JOURNAL  卷: 35   期: 1   页: 34-43   出版年: FEB 2016.

40. A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1；KOREAN JOURNAL OF PARASITOLOGY  卷: 54   期: 1   页: 21-29   出版年: FEB 2016.

41. A pair of transposon‐derived proteins regulate active DNA demethylation in Arabidopsis；EMBO JOURNAL  卷: 35   期: 18   页：2060-2060   出版年: SEP 15 2016.

42. 泛素结合酶RAD6多克隆抗体的制备及初步鉴定；中国处方药 .

• 70601(含kit)-GST融合蛋白纯化磁珠中文说明书-20240527

暂无视频