产品名称 编号 产品说明 单位 单价 加入购物车
GST融合蛋白纯化磁珠 70601-5 5 mL, 10%(v/v), 30-150 μm 瓶 ¥580.00
GST融合蛋白纯化试剂盒 70601-K10 5 mL, 10%(v/v), 30-150 μm 盒 ¥580.00
GST融合蛋白纯化磁珠 70601-100 2×50 mL, 10%(v/v) ,30-150 μm 盒 ¥5000.00
GST融合蛋白纯化磁珠 70601-1000 4×250 mL, 10%(v/v), 30-150 μm 盒 ¥30000.00

海狸GST融合蛋白纯化磁珠是专为高效、快速纯化谷胱甘肽S-转移酶(GST)融合蛋白而设计的一种新型功能化材料，可通过磁性分离方式直接从生物样品中一步纯化出高纯度的目标蛋白，极大地简化纯化工艺和提高纯化效率，适合科研和工业领域便捷地进行GST融合蛋白的纯化。
与传统的柱层析纯化方式相比，采用海狸磁珠纯化GST融合蛋白，无需对粗蛋白样品进行多次长时间的高速离心，也不需要过滤操作；无需控制流速，更不需要昂贵的层析设备。样品与磁珠的特异性结合、洗涤和目标蛋白洗脱变得非常简单、快速、易操作，不存在样品流速控制的瓶颈，对于熟练的操作者来说，在一个小时内就能获得高纯度 的目的蛋白，不仅为研究者简化了流程，节省了时间，降低了成本，并可提高目标蛋白的纯化效率。使用磁珠能轻 松实现多样品平行处理，实现高通量，也可任意放大样品纯化规模。


产品名称BeaverBeads™ GSH
磁珠粒径30μm～150μm
GST融合蛋白结合量可达10mg/mL磁珠（100%）
悬液浓度10%(v/v)磁珠悬液
保存液20%(v/v)乙醇
保存温度4℃～30℃ (长期保存，建议置于4℃～8℃)
化学稳定性常温可耐受70%乙醇、6M盐酸胍、0.1M氢氧化钠、0.1M醋酸1h


应用实例

1. 磁珠与传统层析柱纯化GST融合蛋白对比

注：图中2%、5%、10%、20%和100%指的是分别用谷胱甘肽含量为0.2mM、0.5mM、1mM和10mM的缓冲液对吸附目标蛋白后的磁珠进行洗脱。

2.平行操作稳定性高，可方便实现高通量和大规模蛋白纯化

结果：CV(目标蛋白纯度） = 1.6%，CV（ 目标蛋白载量）= 4.5%。

此外，放大规模纯化样品时，只需同比例增加磁珠用量即可，并不影响目标蛋白的产率和纯度，完全不存在流速和样品体积的限制，海狸磁珠更加适用于大规模蛋白纯化生产。

3.不同批次间产品使用性能稳定，批次间差异小

结果：CV(目标蛋白纯度）= 0.7%，CV（ 目标蛋白载量）= 4.7%

4.可重复使用

结果：同一样品，重复使用5次后，其目标蛋白载量没有明显下降，目标蛋白纯度基本不变。为了保障纯化的质量，建议3~5次后，进行清洗使用处理。

产品特性

1、蛋白纯化快捷

     操作简单，一步纯化即可得到高纯度蛋白；

     方便快捷，一个小时轻松实现蛋白纯化。

2、蛋白纯化灵活

     可同时处理多个样品，实现高通量；

     可轻松实现蛋白浓度和体积的控制，方便后续样品精制；

     可轻松实现对纯化规模的控制；

     对微量和低丰度样品具有良好的纯化效果。

3、蛋白纯化经济

     纯化流程短，蛋白产量及活性高；

     设备简单，减少了设备购买及维护成本，蛋白纯化门槛大大降低；

     纯化时间短，节约了时间成本；

     可同时进行多样品处理，实现自动化操作，节约人工成本；

     能够重复使用。

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