| 产品名称 | 编号 | 产品说明 | 单位 | 单价 | 加入购物车 |
| His-tag蛋白纯化磁珠（IDA-Ni) | 70501-5 | 5 mL, 10%(v/v), 30-150 μm | 瓶 | ¥580.00 | |
| His-tag蛋白纯化磁珠（IDA-Ni) | 70501-100 | 2×50 mL, 10%(v/v), 30-150 μm | 盒 | ¥5000.00 | |
| His-tag蛋白纯化磁珠（IDA-Ni) | 70501-1000 | 4×250mL, 10%(v/v), 30-150 μm | 盒 | ¥30000.00 | |
| His-tag蛋白纯化试剂盒（IDA-Ni) | 70501-K10 | 10次反应试剂盒(5 mL) | 盒 | ¥580.00 | |
| His-tag蛋白纯化磁珠（IDA-Co) | 70502-5 | 5 mL, 10%(v/v), 30-150 μm | 瓶 | ¥580.00 | |
| His-tag蛋白纯化磁珠（IDA-Co) | 70502-100 | 2x50 mL, 10%(v/v), 30-150 μm | 盒 | ¥5000.00 | |
| His-tag蛋白纯化磁珠（IDA-Co) | 70502-1000 | 4x250 mL, 10%(v/v), 30-150 μm | 盒 | ¥30000.00 | |
| His-tag蛋白纯化试剂盒（IDA-Co) | 70502-K10 | 10次反应试剂盒(5 mL) | 盒 | ¥580.00 | |
| His-tag蛋白纯化磁珠（NTA-Ni) | 70521P-5 | 5 mL, 25% (v/v), 10-30 μm | 瓶 | 询价 | |
| His-tag蛋白纯化磁珠（NTA-Ni) | 70521P-10 | 10 mL, 25%(v/v), 10-30 μm | 瓶 | 询价 | |
| His-tag蛋白纯化磁珠（NTA-Ni) | 70521P-100 | 2×50 mL, 25%(v/v), 10-30 μm | 瓶 | 询价 |
BeaverBeads™ His-tag Protein Purification磁珠（组氨酸标签蛋白纯化琼脂糖磁珠）是专为高效、快速纯化组氨酸标签蛋白而设计的一种新型功能化材料，可通过磁性分离方式直接从生物样品中一步纯化出高纯度的目标蛋白，显著地简化了纯化工艺，降低了设备需求，适合科研和工业领域便捷快速地进行组氨酸标签蛋白的纯化工作。
对比传统的过柱层析纯化方式， His-tag Protein Purification磁珠通过磁性分离方式纯化组氨酸标签蛋白，无需对粗蛋白样品进行多次费时费力的离心、过滤步骤，无需装柱和过柱层析，无需昂贵的柱层析设备，在1小时内便能便捷地获得高产量和高纯度的目的蛋白，且能轻松实现多样品平行处理，显著提高了工作效率，大大降低了设备、时间和人工成本.
本产品系列包括镍离子（Nickel）和钴离子（Cobalt）两种金属离子螯合磁珠。它们在目标蛋白结合量及所得目标蛋白纯度上有所差异，一般推荐客户使用镍离子螯合磁珠，大多数情况下能满足客户对蛋白载量和纯度的要求；对纯度要求更高的客户推荐使用钴离子螯合磁珠。
His-tag Protein Purification磁珠广泛适用于细菌、酵母、昆虫和哺乳动物细胞等分泌或胞内表达的可溶性组氨酸标签蛋白以及变性蛋白的纯化。
产品特点
1.可从粗样品直接纯化目标蛋白，极大的缩短纯化时间
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2. 可轻松实现对目标蛋白浓度和体积的控制
通过调节洗脱缓冲液体积的方式，实现对目标蛋白浓度和体积的控制，无蛋白损失，且不存在蛋白变性、沉淀的风险。
3. 一步纯化即可获得高纯度目标蛋白
| 海狸磁珠与市场上进口产品相比，一步纯化即得到同水平高纯度的目标蛋白。一个完整的操作流程即可获得纯度可以达到95％以上的目标蛋白。 |
4.平行操作稳定性高，可方便实现高通量和大规模蛋白纯化
目的蛋白纯度和产量的标准差均<5%。
完全不存在流速和样品体积的限制，适用于高通量和大规模蛋白纯化。
5.可以获得较高的目标蛋白产率
利用海狸磁珠对粗样品进行纯化，一步完整的纯化流程之后，90％以上纯度的目标蛋白，其产率一般达到70～80％。
6.可重复使用，再生工艺简单
由图A、B可知，海狸磁珠反复进行多达六次再生处理，仍能保持较好的目标蛋白结合能力和纯度。
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图A. 再生6次后磁珠结合目标蛋白检测图谱 图B. 再生6次后磁珠的蛋白结合量数据图
7. 镍螯合磁珠与钴螯合磁珠对不同分子量的His-tag蛋白纯化对比
Co离子螯合磁珠蛋白结合量比Ni离子螯合磁珠稍低，但纯度更高；
Co离子螯合磁珠洗涤和洗脱所用咪唑浓度都比Ni离子螯合磁珠低。
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图A.镍和钴螯合磁珠纯化His-tag Lif 蛋白 图B.镍和钴螯合磁珠纯化M-MLv蛋白
（分子量～ 50KD）对比 （分子量～ 70KD）对比
引用文献：
1. Screening, characterization and specific binding mechanism of aptamers against human plasminogen Kringle 5. Bioorganic Chemistry, 2023.
2. MADS-box protein AGL8 interacts with chromatin-remodelling component SWC4 to activate thermotolerance and environment-dependent immunity in pepper. Analytica chimica acta, 2023.
3. Structural basis for the self-recognition of sDSCAM in Chelicerata. Nature communications, 2023.
4. RPL21 interacts with LAMP3 to promote colorectal cancer invasion and metastasis by regulating focal adhesion formation. Cellular & molecular biology letters, 2023.
5. A novel protein elicitor (PeSy1) from Saccharothrix yanglingensis induces plant resistance and interacts with a receptor-like cytoplasmic kinase in Nicotiana benthamiana. Molecular Plant Pathology, 2023.
6. The CsHSFA-CsJAZ6 module mediated high temperature regulates flavonoid metabolism in Camellia sinensis. Plant, cell & environment, 2023.
7. Proteomic analysis of extracellular vesicles from tick hemolymph and uptake of extracellular vesicles by salivary glands and ovary cells. Parasites & vectors, 2023.
8. Pseudorabies Virus Regulates the Extracellular Translocation of Annexin A2 To Promote Its Proliferation. Journal of virology, 2023.
9. Stepwise Optimization of Inducible Expression System for the Functional Secretion of Horseradish Peroxidase in Saccharomyces cerevisiae. Journal of agricultural and food chemistry, 2023.
10. Functional differentiation of two general-odorant binding proteins in Hyphantria cunea (Drury) (Lepidoptera: Erebidae). Pest management science, 2023.
11. 4ENB1 encodes a cellulose synthase 5 that directs synthesis of cell wall ingrowths in maize basal endosperm transfer cells. Plant Cell 2022 03 04;34(3).
12. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency. Cell 2022 May 26;185(11). DOI: doc88.com/p-08661512614974.html.
13. aSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper. PLoS Genet 2022 02;18(2).
14. Characterization of a novel type homoserine dehydrogenase with high oxidation activity from Arthrobacter nicotinovorans. Process Biochemistry 114 (2022) 102–110.
15. Heat shock protein 101 contributes to the thermotolerance of male meiosis in maize. Plant Cell. 2022 Jun 27;koac184. DOI: 10.1093/plcell/koac184/6618537?login=false.
16. Ligand-binding properties of odorant-binding protein 6 in Athetis lepigone to sex pheromones and maize volatiles. Pest Manag Sci 2022 Jan;78(1).
17. Mycoplasma synoviae dihydrolipoamide dehydrogenase is an immunogenic fibronectin/plasminogen binding protein and a putative adhesin. Vet Microbiol 2022 Feb;265.
18. Small Molecules Promote Selective Denaturation and Degradation of Tubulin Heterodimers through a Low-Barrier Hydrogen Bond. Jmedchem, 2022. DOI: 10.1021/acs.jmedchem.2c00379.
19. The Therapeutic Effect of an Anti-TNF-α/HSA/IL-6R Triple-Specific Fusion Protein Under Experimental Septic Conditions. Inflammation 2022 Apr;45(2).
20. Type One Protein Phosphatase Regulates Fixed-Carbon Starvation-Induced Autophagy by Dephosphorylating ATG13a to Facilitate ATG1a-ATG13a Formation in Arabidopsis. bioRxiv, 2022.
21. Enhanced Thermostability of Pseudomonas nitroreducens Isoeugenol Monooxygenase by the Combinatorial Strategy of Surface Residue Replacement and Consensus Mutagenesis.Catalysts, 2021, 11.DOI:10.3390/catal11101199.
22. Corrigendum to: Proteomics analysis reveals the importance of transcriptional regulator slyA in regulation of several physiological functions in Aeromonas hydrophila.Journal of Proteomics, 2023, Oct, 24(250). DOI: 10.1016/j.jprot.2021.104390.
23. Bluetongue Virus Non-structural Protein 3 (NS3) and NS4 Coordinatively Antagonize Type Ⅰ Interferon Signaling by Targeting STAT1.Veterinary Microbiology, 2021, 254:108986.DOI:10.1016/j.vetmic.2021.108986.
24. Fluorescent assay for quantitative analysis of trimethylamine N-oxide. Analytical Methods, 2021, 13(12): 1527-1534. DOI: 10.1039/d0ay02353a.
25. Administration of fusion cytokines induces tumor regression and systemic antitumor immunity. MedComm, 2021, 2(2): 256-268. DOI: 10.1002/mco2.68.
26. Characterization of a Novel Type Homoserine Dehydrogenase Only with High Oxidation Activity from Arthrobacter nicotinovorans. bioRxiv, 2021. DOI: 10.1101/2021.02.09.430557.
27. Construction and directional evolution of antienrofloxacin ScFv antibody for the immunoassay of fluoroquinolone. Research Square. DOI: 10.21203/rs.3.rs-310107/v2.
28. SARS-COV-2 RBD Oral Vaccine Boosted by Mucosal Immune Adjuvant LTB26 via DCs and B Cells Activation in Mice. bioRxiv, 2021. doi.org/10.1101/2020.04.06.025981.
29. The therapeutic effect of a novel anti-TNF-α/IL-6R triple-specific fusion protein under experimental septic condition. Research Square. DOI: 10.21203/rs.3.rs-298423/v1.
30. Overexpression of a cysteine proteinase inhibitor gene from Jatropha curcas confers enhanced tolerance to salinity stress；ELECTRONIC JOURNAL OF BIOTECHNOLOGY 卷: 18 期: 5 页: 368-375 出版年: SEP 2015
31. Production and Stabilization of an Integrin Binding Moiety of Complement Component 3；MOLECULAR BIOLOGY 卷: 49 期: 5 页: 723-727 出版年: SEP 2015
32. The role of semaphorin 4D as a potential biomarker for antiangiogenic therapy in colorectal cancer；ONCOTARGETS AND THERAPY 卷: 9 页: 1189-1204 出版年: 2016
33. 血红铆钉菇免疫调节蛋白基因克隆及其生物信息学分析和重组表达；沈阳农业大学学报
34. Innovation Spaces in Asia: Entrepreneurs, Multinational Enterprises and Policy；
35. 登革病毒四型联合重组包膜蛋白III 区的表达和免疫原性鉴定*；中国生物工程杂志
36. Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1；INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 卷: 17 期: 9 文献号: 1419 出版年: SEP 2016
37. 人C-Src 蛋白酪氨酸激酶真核表达, 纯化及活性检测；生物技术通报
38. 人酪氨酸蛋白激酶Lyn的真核表达、纯化及鉴定；生物技术
39. 一个麻风树Kunitz型蛋白酶抑制剂基因的克隆和鉴定；四川大学学报(自然科学版)
40. 锌离子依赖金属蛋白酶1(Zmp1)抑制小鼠RAW264.7细胞增殖以及吞噬体-溶酶体的融合；细胞与分子免疫学杂志 41. A Novel Assay for Screening Inhibitors Targeting HIV Integrase LEDGF/p75 Interaction Based on Ni2+ Coated Magnetic Agarose Beads；SCIENTIFIC REPORTS 卷: 6 文献号: 33477 出版年: SEP 16 201642. 42. Eukaryotic expression,protein purification and identification of human tyrosine-protein kinase Lyn；
43. The role of semaphorin 4D as a potential biomarker for antiangiogenic therapy in colorectal cancer；ONCOTARGETS AND THERAPY 卷: 9 页: 1189-1204 出版年: 2016
44. Design, Recombinant Fusion Expression and Biological Evaluation of Vasoactive Intestinal Peptide Analogue as Novel Antimicrobial Agent；Molecules 2017, 22, 1963; DOI:10.3390/molecules22111963
45. The thermoduric effects of site-directed mutagenesis of proline and lysine on dextransucrase from Leuconostoc mesenteroides 0326；International Journal of Biological Macromolecules，DOI：10.1016/j.ijbiomac.2017.10.023
46. GR1-like gene expression in Lycium chinense was regulated by cadmium-induced endogenous jasmonic acids accumulation；Plant Cell Rep (2017) 36:1457–1476, DOI:10.1007/s00299-017-2168-2
47. Development of a peptide ELISA to discriminate vaccine-induced immunity from natural infection of hepatitis A virus in a phase IV study；Eur J Clin Microbiol Infect Dis (2017) 36:2165–2170, DOI:10.1007/s10096-017-3040-6
